A novel AAT-deletion mutation in the coding sequence of the BCO2 gene in yellow-fat rabbits

作者：Strychalski Janusz^*; Brym Pawel; Czarnik Urszula; Gugolek Andrzej

来源：Journal of Applied Genetics, 2015, 56(4): 535-537.

DOI：10.1007/s13353-015-0290-9

• BCO2
• Carotenoids
• Deletion mutation
• Rabbits
• Yellow fat

The carcasses of yellow-fat rabbits may be attractive to modern consumers, because they have a relatively high content of biologically active compounds. One of the main candidate genes associated with the yellow-fat trait is beta-carotene 9',10'-oxygenase (BCO2). This study is the first report of the novel AAT-deletion mutation at codon 248 of the BCO2 gene, which has been found in homozygous yellow-fat rabbits. The deletion mutation, located at the beginning of exon 6, results in the absence of asparagine in protein. We also developed a PCR-RFLP test that supports intravital genotyping of indel polymorphism based on genomic DNA.

• 出版日期2015-11