Demineralized Bone Particle Impregnated Poly(L-Lactide-co-Glycolide) Scaffold for Application in Tissue-Engineered Intervertebral Discs

作者：Kim Soon Hee; Song Jeong Eun; Lee Dongwon; Khang Gilson^*

来源：Journal of Biomaterials Science, Polymer Edition, 2012, 23(17): 2153-2170.

DOI：10.1163/092050611X611657

• Demineralized bone particle
• poly(L-lactide-co-glycolide)
• hybrid scaffold
• intervertebral disc

Demineralized bone particle (DBP) contains powerful bioactive molecules that facilitate new bone or cartilage growth. We developed hybrid scaffolds of poly(L-lactide-co-glycolide) (PLGA) with various concentrations of DBP (DBP/PLGA), of which phenotypes on intervertebral disc (IVD) cells were investigated. The hybrid scaffold has a cylindrical donut shape with two distinct parts; the inner is for the nucleus pulposus (NP) and the outer is for annulus fibrosus (AF). Rabbit NP and AF cells were seeded into the inner and outer regions of the DBP/PLGA scaffolds separately. Disc cell viability in DBP/PLGA scaffolds was superior to pure PLGA scaffold and increased with increasing DBP concentration. In vitro-and in vivo-formed tissues were characterized by RT-PCR, Safranin-O, Masson%26apos;s trichrome staining and immunohistochemical staining for type-I and type-II collagen. DBP/PLGA hybrid scaffolds revealed more active expression of disc phenotypes, as characterized by protein and mRNA expression, than the PLGA control. This study provides valuable information for potential disc replacement using DBP and PLGA.

• 出版日期2012