Diarylheotanoid from Alnus hirsuta Improves Glucose Metabolism via Insulin Signal Transduction in Human Hepatocarcinoma (HepG2) Cells

作者：Hu Weicheng^*; Wang Myeong Hyeon

来源：Biotechnology and Bioprocess Engineering, 2011, 16(1): 120-126.

DOI：10.1007/s12257-010-0311-9

• antidiabetic
• Alnus hirsuta
• diarylheptanoid
• insulin receptor

Alnus hirsuta is a deciduous, broad-leaved tree that thrives in damp locations, and the bark of the Alnus species has been used in traditional oriental medicine as a remedy for hemorrhage, fever, diarrhea, and alcoholism. Two diarylheptanoids, 1,7-bis-(3,4-dihydroxyphenyl)-heptane-3-one-5-O-beta-D-xylopyranoside (Compound I) and 1,7-bis-(3,4-dihydroxyphenyl)-heptane-5-O-beta-D-xylopyranoside (Compound II), were isolated from the stem bark of A. hirsuta. In the present study, the investigations of two diarylheptanoids on insulin signaling were performed in insulin-responsive human hepatocarcinoma (HepG2) cells co-treated with high glucose. As the results, the insulin metabolic effect of glucose uptake was inhibited by high glucose. However, treatment of Compound I enhanced the glucose uptake ability in HepG2 cells, whereas, Compound II did not affect glucose incorporation noticeably. In addition, we demonstrated that treatment of HepG2 cells with Compound I improved the mRNA expression level of insulin receptor (IR) and insulin receptor substrate-1 (IRS-1).

• 出版日期2011-2