Heme iron state in various oxyhemoglobins probed using Mossbauer spectroscopy with a high velocity resolution

作者：Oshtrakh M I^*; Berkovsky A L; Kumar A; Kundu S; Vinogradov A V; Konstantinova T S; Semionkin V A

来源：Biometals, 2011, 24(3): 501-512.

DOI：10.1007/s10534-011-9428-3

• Heme iron
• Oxyhemoglobins
• Mossbauer spectroscopy with a high velocity resolution
• Quadrupole splitting

A comparative study of oxyhemoglobins from pig, rabbit, normal human and patients with blood system malignant diseases was performed using Mossbauer spectroscopy with a high velocity resolution at 90 K. Mossbauer spectra were fitted with the help of two models: using one quadrupole doublet (model of equivalent iron electronic structure in alpha- and beta-subunits of hemoglobins) and superposition of two quadrupole doublets (model of non-equivalent iron electronic structure in alpha- and beta-subunits of hemoglobins). The results obtained using both models demonstrated small variations of hyperfine parameters that were related to the heme iron state variation in different hemoglobins. These results were compared with structural and functional differences of the hemoglobins investigated.

• 出版日期2011-6