An Integrated Giant Magnetoresistance Microfluidic Immuno-sensor for Rapid Detection and Quantification of D-dimer

作者：Gao Yu-Zhe; Zhang Lei; Huo Wei-Song; Shi Stone; Lian Jie; Gao Yun-Hua

来源：Chinese Journal of Analytical Chemistry, 2015, 43(6): 802-807.

DOI：10.1016/S1872-2040(15)60827-1

• Giant magnetoresistance
• Microfluidic immuno-sensor
• D-dimer
• Magnetic particles

A method for rapid detection and quantification of D-dimer as a biomarker of thrombosis was developed by utilizing giant magnetoresistance (GMR) sensor integrated with microfluidic technique and 100 nm magnetic particles (MPs) as signal probes. The MPs binding on the chip through specific immune reaction were measured by the GMR sensor and the concentration of D-dimer was obtained at the same time. The detection range of this method was from 5 ng mL(-1) to 6500 ng mL(-1), the detection limit was 5 ng mL(-1), the intra relative standard deviation was less than 12% and the inter relative standard deviation was less than 14%. The whole analysis process could be completed within 9 min, and a good linear correlation between the results of GMR biosensor and Sysmex CA1500 was obtained. The method had the advantages such as high sensitivity, fast detection and good accuracy.

• 出版日期2015-6
• 单位中国人民公安大学; 中国科学院大学; 中国科学院理化技术研究所