Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds

作者：Arecchi Alessandra; Scampicchio Matteo; Drusch Stephan; Mannino Saverio^*

来源：Analytica Chimica Acta, 2010, 659(1-2): 133-136.

DOI：10.1016/j.aca.2009.11.039

• Phenols
• Biosensor
• Nanofiber
• Electrospinning
• Nylon
• Tyrosinase

A tyrosinase-modified electrode is described to be used as amperometric biosensor for the detection of phenolic compounds in food. The enzyme has been immobilized by drop-coating on a glassy carbon electrode covered by a polyamidic nanofibrous membrane prepared by electrospinning. With respect to others, the selectivity of the designed tyrosinase-biosensor resulted modified by the presence of the nanostructured coating which seems to affect the permeability of phenols as a function of the pH of the solution and of their dissociation constants. The biosensor exhibits a response time of 16 s, a detection limit of 0.05 mu M, and a linearity up to 100 mu M (slope: -304 nA mu M(-1); intercept: -191 nA. r(2) = 0.996, n = 19). Among others. it can be successfully used for monitoring in real time the release kinetics of phenols encapsulated in polymeric microcapsules.

• 出版日期2010-2-5