Assessment of TNFA polymorphisms at positions-857 and-863 in Polish peptic ulcer patients

作者：Salagacka Kubiak Aleksandra; Zebrowska Marta; Jelen Agnieszka; Mirowski Marek; Balcerczak Ewa^*

来源：Advances in Medical Sciences, 2016, 61(1): 164-168.

DOI：10.1016/j.advms.2015.12.002

• Genetic polymorphism
• Peptic ulcer
• Restriction fragment length polymorphism
• Tumor necrosis factor-alpha

Purpose: Peptic ulceration connected with chronic inflammation in gastrointestinal mucosa could be induced by Helicobacter pylori infection. Tumor necrosis factor alpha (TNF-alpha) encoded by TNFA gene is a key mediator in the inflammation process. There are several polymorphisms in the promoter of TNFA influencing its transcriptional activity. -857C>T (rs1799724) and -863C>A (rs1800630) substitutions may be responsible for increased TNFA transcription and TNF-alpha production. The association of these two polymorphisms with peptic ulceration and the development of H. pylori infection in peptic ulcer patients in Poles were evaluated.
Material and methods: Polymorphisms were assessed by PCR-RFLP in 203 peptic ulcer patients. H. pylori infection was confirmed by rapid urease test. The results of genotyping were compared with those obtained for 248 healthy Polish individuals.
Results: There were no significant differences in genotype and allele frequencies for both investigated polymorphisms between peptic ulcer patients and healthy individuals. No associations between frequencies of particular genotypes and alleles for both SNPs and the presence of H. pylori infection in peptic ulcer patients and in subgroups of peptic ulcer women and men were confirmed.
Conclusions: The investigated SNPs are not risk factors for peptic ulcer development. They are not risk factors for H. pylori infection in ulcer patients.

• 出版日期2016-3