A selective sensing membrane for the determination of tetracycline with heptakis (2,6-di-O-isobutyl)-beta-cyclodextrin as the substrate

作者：Yang, RH; Wang, KM^*; Xiao, D; Yang, XH; Zhang, LD

来源：Microchemical Journal, 2000, 64(3): 213-220.

DOI：10.1016/S0026-265X(00)00010-2

When heptakis (2,6-di-O-isobutyl)-beta-cyclodextrin(DOB-beta-CD) is immobilized in a plasticized poly vinyl chloride (PVC) membrane, it extracts tetracycline (TC) from the sample solution into the organic membrane phase to form a complex of DOB-beta-CD and TC. Since the complex formation results in an enhancement of fluorescence intensity of TC at 506 nm, the chemical recognition process can be directly translated into an optical signal. The maximum response of the sensitive membrane for TC was obtained in 0.2 mol/l KH2PO4-KOH buffer solution (pH 8.01). In the optimum conditions described, the proposed sensor responds linearly in the measuring range of 2.00 x 10(-6) mol/l to 4.00 x 10(-4) mol/l, and has a detection limit of 8.00 x 10(-7) mol/l. The response time of the sensor is within 2.0 min. In addition to high reproducibility and reversibility, the sensor also exhibits good selectivity over some common pharmaceutical species and some common organic and inorganic compounds.

• 出版日期2000-5
• 单位湖南大学