Application of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella pyogenes isolated from various origins

作者：Abdulmawjood A^*; Wickhorst J; Hashim O; Sammra O; Hassan A A; Alssahen M; Laemmler C; Prenger Berninghoff E; Klein G

来源：Molecular and Cellular Probes, 2016, 30(4): 205-210.

DOI：10.1016/j.mcp.2016.05.003

• Trueperella pyogenes
• Loop-mediated isothermal amplification
• LAMP
• Real-time fluorometer

In the present study 28 Trueperella pyogenes strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene cpn60 encoding chaperonin. No cross reaction could be observed with control strains representing four species of genus Trueperella and seven species of closely related genus Arcanobacterium. The present cpn60 LAMP assay might allow a reliable and low cost identification of T. pyogenes also in laboratories with less specified equipment.

• 出版日期2016-8