Microimmunoanalysis on standard compact discs to determine low abundant compounds

作者：Morais Sergi; Carrascosa Javier; Mira David; Puchades Rosa; Maquieira Angel^*

来源：Analytical Chemistry, 2007, 79(20): 7628-7635.

DOI：10.1021/ac070328b

High-density competitive indirect microimmunoassays were performed in both sides of compact discs by direct absorption of immunoreagents on polyearbonate surface, using gold- or enzyme-labeled immunoglobulins as tracers for displaying the immunoreaction. The operational principle is based on the use of a low-reflectivity compact disc as analytical platform that allows the reflection/transmission (30/70%) of the CD reader laser beam (lambda 780 nm). The reflected light is used to scan the disc track keeping it in movement. The transmitted light is detected by a planar photodiode integrated on the CD drive. The variation of the optical transmission of the light caused by the immunoreaction products is related to the sample concentration. As a proof of concept, low abundant compounds, commonly used as pesticides, were detected in a 60-min total assay time, with a limit of detection ranging from 0.02 to 0.62 mu g/L for 2,4,5-TP, chlorpyriphos, and metolachlor. The obtained results show the enormous prospective of compact discs in combination with CD players for multiresidue and drug discovery applications.

• 出版日期2007-10-15