Evaluation of Remnant Liver Function using C-13-Breath Tests in a Rat Model of 70% Partial Hepatectomy

作者：Miura Yasuyuki^*; Washizawa Naohiro; Urita Yoshihisa; Imai Tsunehiko; Kaneko Hironori

来源：Hepato-Gastroenterology, 2012, 59(114): 311-316.

DOI：10.5754/hge11431

• C-13-breath test
• C-13-phenylalanine
• C-13-leucine
• 70% Partial hepatectomy
• (CO2)-C-13
• Ki-67
• Liver metabolism
• Early after hepatectomy

Background/Aims: A C-13 breath test was applied to evaluate the early stage of postoperative liver function after hepatectomy. It was examined in comparison with functional and morphological recovery. Methodology: Rats were subjected to 70% partial hepatectomy (H group) and control group (S group). Expired air, blood and remnant liver tissue were collected at 12, 24, 48 and 72h, and 7 and 14 days postoperatively. Phenylalanine and leucine were used as the C-13-labeled substrates. The (CO2)-C-13 was collected until 120min. The mean values of the H and S groups were compared using the highest mean level of (CO2)-C-13 excreted in breath (Cmax). The liver regenerative ability was evaluated by Ki-67. Results: In the C-13-phenylalanine breath test, the Cmax value tended to be lower for the H group 12h postoperatively (p=0.06). Cmax was significantly lower for the H group 24h postoperatively (p%26lt;0.05). Both groups showed equal recovery at 48h postoperatively. In the C-13-leucine breath test, both groups had no significant difference after hepatectomy. The peak of Ki-67 positive rate is estimated to be at 52.8h postoperatively, meaning that the time difference was only approximately 5h between the peak time of Ki-67 positive rate and the C-13-phenylalanine breath test. Conclusions: These results strongly support that the C-13-phenylalanine breath test can become a non-invasive indicator to evaluate the early stage of postoperative liver function after hepatectomy.

• 出版日期2012-4