Analysis of chlorpyrifos and TCP residues in agricultural soil and apples by HPLC

作者：Zhang, Qingming; Zhu, Lusheng^*; Han, Chang; Wang, Jun; Xie, Hui; Wang, Jinhua; Sun, Shujuan

来源：Journal of Food Agriculture and Environment, 2011, 9(1): 659-662.

• Organophosphate insecticides
• metabolite
• solid-phase extraction
• HPLC

In the present work, a method for the analysis of the residues of chlorpyrifos and its toxic metabolite 3,5,6-trichloro-2-pyridinol (TCP) in agricultural soil and apples was developed. The residues of chlorpyrifos and TCP were extracted from agricultural soil and apple samples by shaking followed clean-up by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) coupled with detection of high-performance liquid chromatography (HPLC). Under the optimum conditions, the average recovery of chlorpyrifos and TCP with concentrations of 0.1-1.0 mg kg(-1) from soil samples was 92.9-100.3% and 95.7-97.6%, respectively, and the relative standard deviation was 3.7-14.8% and 2.8-13.5%, respectively. The average recovery of chlorpyrifos and TCP from apple samples was 88.9-93.3% and 91.5-97.5%, respectively, and the relative standard deviation was 3.6-15.1% and 2.3-2.3%, respectively. In conclusion, the proposed method can be successfully applied for the determination of chlorpyrifos and TCP residues in soil and apple samples.

• 出版日期2011-1
• 单位青岛农业大学; 山东农业大学