MicroRNA-351-5p mediates skeletal myogenesis by directly targeting lactamase- and is regulated by lnc-mg

作者：Du, Jingjing; Zhang, Peiwen; Zhao, Xue; He, Jin; Xu, Yan; Zou, Qin; Luo, Jia; Shen, Linyuan; Gu, Hao; Tang, Qianzi; Li, Mingzhou; Jiang, Yanzhi; Tang, Guoqing; Bai, Lin; Li, Xuewei; Wang, Jinyong; Zhang, Shunhua^*; Zhu, Li^*

来源：The FASEB Journal, 2019, 33(2): 1911-1926.

DOI：10.1096/fj.201701394RRR

• ceRNA
• C2C12 cells
• proliferation
• differentiation

Skeletal muscle is an important and complex organ with a variety of functions in humans and animals. Skeletal myogenesis is a multistep and complex process, and increasing evidence suggests that microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) play critical roles in skeletal myogenesis. In this study the expression of miR-351-5p is dynamically regulated during skeletal myogenesis in vitro and in vivo. Cell-counting kit-8, qRT-PCR, and EdU immunofluorescence analysis showed that miR-351-5p overexpression promoted the proliferation and inhibited the differentiation of C2C12 myoblast, whereas inhibition of miR-351-5p had the opposite effect. In addition, miR-351-5p mediated the regulation of muscle fiber type transition in vivo. In vitro, loss of miR-351-5p in muscle tissues promoted muscle hypertrophy and increased slow-twitch fibers in the gastrocnemius muscles of mice. Luciferase reporter assay and functional analyses demonstrated that lactamase (LACTB) is a direct target of miR-351-5p involved in the regulation of skeletal myogenesis. Expression levels of a myogenesis-associated lncRNA (lnc-mg) correlated negatively with miR-351-5p and positively with LACTB during C2C12 myoblast proliferation and differentiation. Further analyses showed that lnc-mg acted as a molecular sponge for miR-351-5p, demonstrating its involvement in the negative regulation of LACTB by miR-351-5p during skeletal myogenesis. These findings indicate that miRNA-351-5p functions in skeletal myogenesis by targeting LACTB and is regulated by lnc-mg, supporting the role of the competing endogenous RNA network in skeletal myogenesis.Du, J., Zhang, P., Zhao, X., He, J., Xu, Y., Zou, Q., Luo, J., Shen, L., Gu, H., Tang, Q., Li, M., Jiang, Y., Tang, G., Bai, L., Li, X., Wang, J., Zhang, S., Zhu, L. MicroRNA-351-5p mediates skeletal myogenesis by directly targeting lactamase and is regulated by lnc-mg.

• 出版日期2019-2
• 单位四川农业大学; 重庆市畜牧科学院