Now you see it, now you don%26apos;t: The challenge of detecting, monitoring and conserving ectomycorrhizal fungi

作者：Van der Linde Sietse^*; Holden Elizabeth; Parkin Pamela I; Alexander Ian J; Anderson Ian C

来源：Fungal Ecology, 2012, 5(5): 633-640.

DOI：10.1016/j.funeco.2012.04.002

• Below-ground detection
• Ectomycorrhizal (ECM) fungi
• Fungal conservation
• Species persistence
• Species-specific PCR
• Stipitate hydnoid fungi

Fungi are a vital component of ecosystem biodiversity, but spend most of their lives hidden from view. Monitoring ectomycorrhizal (ECM) fungi has mostly relied on the abundance and distribution of above-ground sporocarps without consideration of their below-ground vegetative mycelium. Molecular methods may provide the means of obtaining this information and allow a more accurate determination of their possible decline and threat of extinction. Stipitate hydnoid sporocarp occurrence was recorded and mapped for 9 yr at two sites in Scotland, UK. Soil samples were collected at locations where sporocarps of Hydnellum aurantiacum, Hydnellum caeruleum, Phellodon niger or Sarcodon glaucopus had occurred 1-4 yr previously. Species-specific DNA was detected at all sporocarp locations and RNA was detected at 75 % of the locations indicating that these species remained below-ground and viable at the majority of locations for at least 4 yr in the absence of sporocarps.

• 出版日期2012-10