Unique Fragmentation of Singly Charged DEST Cross-Linked Peptides

作者：He, Yi; Lauber, Matthew A.; Reilly, James P.^*

来源：Journal of the American Society for Mass Spectrometry, 2012, 23(6): 1046-1052.

DOI：10.1007/s13361-012-0372-4

• Cross-linking
• Fragmentation pattern
• Collisional induced dissociation
• MALDI ion trap

It has previously been shown that when cross-linking reagent diethyl suberthioimidate (DEST) reacts with primary amines of proteins to yield amidinated residues, the primary amines retain their high basicity, and cross-linked species can be enriched by strong cation exchange. It is now demonstrated that collisional activation of singly-charged DEST cross-linked peptide ions leads to preferential cleavage at the cross-linked sites. The resulting product ions facilitate the detection and identification of cross-linked peptides.

• 出版日期2012-6