Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea

作者：Buhr Ethan D; Yue Wendy W S; Ren Xiaozhi; Jiang Zheng; Liao Hsi Wen Rock; Mei Xue; Vemaraju Shruti; Nguyen Minh Thanh; Reed Randall R; Lang Richard A; Yau King Wai^*; Van Gelder Russell N

来源：Proceedings of the National Academy of Sciences, 2015, 112(42): 13093-13098.

DOI：10.1073/pnas.1516259112

• OPN5
• photoentrainment
• circadian rhythm
• retina
• cornea

The molecular circadian clocks in the mammalian retina are locally synchronized by environmental light cycles independent of the suprachiasmatic nuclei (SCN) in the brain. Unexpectedly, this entrainment does not require rods, cones, or melanopsin (OPN4), possibly suggesting the involvement of another retinal photopigment. Here, we show that the ex vivo mouse retinal rhythm is most sensitive to short-wavelength light but that this photoentrainment requires neither the short-wavelength-sensitive cone pigment [S-pigment or cone opsin (OPN1SW)] nor encephalopsin (OPN3). However, retinas lacking neuropsin (OPN5) fail to photoentrain, even though other visual functions appear largely normal. Initial evidence suggests that OPN5 is expressed in select retinal ganglion cells. Remarkably, the mouse corneal circadian rhythm is also photoentrainable ex vivo, and this photoentrainment likewise requires OPN5. Our findings reveal a light-sensing function for mammalian OPN5, until now an orphan opsin.

• 出版日期2015-10-20