Monitoring of antigen-specific CD8 T cells in patients with type 1 diabetes treated with antiCD3 monoclonal antibodies

作者：Cernea Simonal; Herold Kevan C^*

来源：Clinical Immunology, 2010, 134(2): 121-129.

DOI：10.1016/j.clim.2009.09.005

• Type 1 diabetes
• CD8 T cells
• Tetramer
• AntiCD3 monoclonal antibody

The way in which anti-CD3 monoclonal antibodies (mAbs) modify human immune responses in type 1 diabetes (T1DM) is not known. We prepared a panel of Class 1 HLA-A2.1 tetramers with peptides from diabetes-associated antigens and studied the frequency and phenotype of the cells in patients with T1DM and blood donors and in patients treated with anti-CD3 mAb (Teplizumab). More patients with T1DM showed positive staining for at least 1 tetramer using frozen and fresh samples (p < 0.05). Three months following treatment with anti-CD3 mAb, the proportion of GAD65- and InsB-peptide reactive CD8+ T cells increased (p < 0.05). The phenotype of these cells was modulated from naive to effector memoryRA+. We concludethat Class 1 MHC tetramers can identify antigen specific CD8+ T cells in patients with T1DM. The frequency of certain specificities increases after treatment with anti-CD3 mAb. Their modulated phenotype may have functional consequences for their pathogenicity.

• 出版日期2010-2