A Simple, Rapid Atmospheric Pressure Chemical Ionization Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of 25-Hydroxyvitamin D2 and D3

作者：Garg Uttam^*; Munar Ada; Frazee Clinton III; Scott David

来源：Journal of Clinical Laboratory Analysis, 2012, 26(5): 349-357.

DOI：10.1002/jcla.21530

• vitamin D
• 25-hydroxyvitamin D2
• 25-hydroxyvitamin D3
• tandem mass spectrometry

Vitamin D plays a vital role not only in bone health but also in pathophysiology of many other body functions. In recent years, there has been significant increase in testing of 25-hydroxyvitamin D (25-OH vitamin D), a marker of vitamin D deficiency. The most commonly used methods for the measurement of 25-OH vitamin D are immunoassays and liquid chromatography tandem mass spectrometry (LC-MS-MS). Since immunoassays suffer from inaccuracies and interferences, LC-MS-MS is a preferred method. In LC-MS-MS methods, 25-OH vitamin D is extracted from serum or plasma by solid-phase or liquid-phase extraction. Because these extraction methods are time consuming, we developed an easy method that uses simple protein precipitation followed by injection of the supernatant to LC-MS-MS. Several mass-to-charge (m/z) ratio transitions, including commonly used transitions based on water loss, were evaluated and several tube types were tested. The optimal transitions for 25-OH vitamin D2 and D3 were 395.5 > 269.5 and 383.4 > 257.3, respectively. The reportable range of the method was 1100 ng/mL, and repeatability (within-run) and within-laboratory imprecision were <4% and <6%, respectively. The method agreed well with the solid-phase extraction methods. J. Clin. Lab. Anal. 26:349-357, 2012.

• 出版日期2012