A Novel delta-Hemolysis Screening Method for Detecting Heteroresistant Vancomycin-Intermediate Staphylococcus aureus and Vancomycin-Intermediate S-aureus

作者：Cafiso V; Bertuccio T; Spina D; Purrello S; Blandino G; Stefani Stefania^*

来源：Journal of Clinical Microbiology, 2012, 50(5): 1742-1744.

DOI：10.1128/JCM.06307-11

We assessed a new screening method, based on delta-hemolysin production in the presence of 6 mg/liter vancomycin, to distinguish heteroresistant vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) from vancomycin-susceptible S. aureus (VSSA). On 37 clinical methicillin-resistant S. aureus (MRSA) isolates, hVISA and VISA displayed no delta-hemolysis whereas VSSA displayed strong delta-hemolysis, showing 91.6% sensitivity. These data, supported by real-time reverse transcription PCR (real-time RT-PCR) highlighting an hld downregulation, i.e., VSSA%26gt;hVISA%26gt;VISA, define this new assay as a valid screening method.

• 出版日期2012-5