Assessment of polyphenolic compounds, in vitro antioxidant and anti-inflammation properties of Securidaca longepedunculata root barks

作者：Muanda Francois Nsemi^*; Dicko Amadou; Soulimani Rachid

来源：Comptes Rendus Biologies, 2010, 333(9): 663-669.

DOI：10.1016/j.crvi.2010.07.002

• Polyphenolic compounds
• Flavonoids
• Free radicals
• RP-HPLC
• Medicinal plants
• Antioxidant activity

The present study was carried out to evaluate the antioxidant activities of the root bark extract of Securidaca longepedunculata. This plant material is commonly used in folk medicine in several parts in the world. The bark extracts of S. longepedunculata were evaluated for their total phenols, flavonoids, anthocyanins, tannins content and total antioxidant capacity. The compounds were identified and quantified both by RP-HPLC and UV spectrophotometer; the antioxidant capacity was assessed by ABTS and DPPH tests and expressed as IC(50). The total phenolic compounds determinate was 9.86 mg gallic acid equivalents/g dw, the total flavonoid contents was 5.85 mg catechin equivalents/g dw, the total anthocyanin contents was 0.032 mg cyanidin-3-glycosyl equivalents/g dw and the condensed tannins content were 1.03 mg catechin equivalents/g dw. The major compound identified using RP-HPLC was quercetin (0.98 mg/ml). The IC(50) value reached 5.5 mu g/ml, revealing that the root barks of S. longepedunculata have a very high antioxidant and anti-inflammation properties.

• 出版日期2010-9