Detection and isolation of p-nitrophenol-lowering bacteria from intestine of marine fishes caught in Japanese waters

作者：Kuda Takashi^*; Kyoi Daisuke; Takahashi Hajime; Obama Kazuhiro; Kimura Bon

来源：Marine Pollution Bulletin, 2011, 62(8): 1622-1627.

DOI：10.1016/j.marpolbul.2011.06.015

• p-Nitrophenol
• Bacteria
• Fish intestine
• Lactococcus lactis sp.

To determine the existence of p-nitrophenol (PNP)-lowering bacteria in intestine of Japanese coastal fish, the gastro-intestinal contents were incubated in Brain Heart Infusion (BHI) broth and minimal medium (mm) broths containing 1 mmol/L PNP at 30 degrees C for 7 days. Among 26 samples of 19 fish species, 17 samples showed a decrease in PNP of 0.5-0.8 mmol/L in BHI broth, but no decrease was shown in MM broth. Eighteen PNP-lowering bacterial strains were isolated from four fishes. All of the strains were identified as Lactococcus lactis subsp. lactis. Three L. lactis strains JS1-3 isolated from Japanese seabass Lateolabrax japonicus showed the highest PNP-lowering activity (0.44 mmol/L). Optimum temperature and pH for the growth and PNP decreasing corresponded with the marine environment. These results suggested that marine fishes have PNP decreasing bacteria in their intestine. These bacteria might protect host fish from toxicities of PNP and PNP related compounds.

• 出版日期2011-8