Distinct Expression Profiles in Type III Collagen and alpha-Smooth Muscle Actin between Human Dental Pulp Stem Cells and Human Mesenchymal Stem Cells

作者：Lee Eunkyung; Lee Jungsun; Son Youngsook^*

来源：Tissue Engineering and Regenerative Medicine, 2008, 5(4-6): 559-564.

• Dental pulp stem cell
• mesenchymal stem cell
• type III collagen
• alpha-smooth muscle actin
• multipotency

In this study, we characterized adult human dental pulp stem cells(hDPSC) in comparison with human mesenchymal stem cells(hMSC). hDPSCs revealed their capacity to form fibroblastic colony forming unit during the extended cell culture. Expression profile of surface markers of hDPSC was similar to that of hMSC. Both cells strongly expressed CD29 and CD44 but did not express CD105, CD117 and CD166, CD34 and CD45. However their expression profiles of type III collagen and a-smooth muscle acti(a-SMA)were quite distinct between hMSC and hDPSC. hMSC were strongly positive in type III collagen expression but were weakly positive in a-SMA. In contrast, hDPSC were very weak in type III collagen expression and strongly positive in a-SMA expression. hDPSCs were also successfully differentiated into osteoblasts or adipocytes under differentiation inducing specific conditions, suggesting their multipotency. Therefore even with overall similarity in the surface marker expression, hDPSC may be clearly distinguished from hMSC based on the expression profiles of type III collagen and a alpha-SMA.

• 出版日期2008-12