Drospirenone induces decidualization in human eutopic endometrial stromal cells and reduces DNA synthesis of human endometriotic stromal cells

作者：Miyashita Mariko; Koga Kaori^*; Izumi Gentaro; Makabe Tomoko; Hasegawa Akiko; Hirota Yasushi; Hirata Tetsuya; Harada Miyuki; Fujii Tomoyuki; Osuga Yutaka

来源：Fertility and Sterility, 2015, 104(1): 217-+.

DOI：10.1016/j.fertnstert.2015.03.023

• Drospirenone
• endometriosis
• endometrium
• decidualization
• oral contraceptive(s)

Objective: To investigate the in vitro effect of drospirenone on human eutopic endometrial (EuSC) and ectopic endometriotic stromal cells (EcSC).
Design: Comparative and laboratory study. The experimental procedures were approved by the Institutional Review Board of the University of Tokyo (registration no. 0324-4).
Setting: University research laboratory.
Patients(s): Eight patients undergoing hysterectomy for benign gynecologic disease and 19 patients undergoing cystectomy or adnectomy for endometriosis. Intervention(s): EuSC and EcSC were treated with drospirenone.
Main Outcome Measure(s): For the analysis of decidualization of EuSC, cells were observed using microscopy, and the production of PRL was measured using enzyme immunoassay. For the analysis of DNA synthesis of EcSC, 5-bromo-2'-deoxyuridine incorporation was measured by ELISA. Cells in apoptosis were detected and measured by flow cytometry.
Result(s): Drospirenone induced decidualization in EuSC, and the induction was negated by RU486. Drospirenone reduced DNA synthesis on EcSC, and this reduction was negated by RU486 or P receptor silencing, but not by aldosterone or mineralocorticoid receptor silencing. Drospirenone did not cause EcSC to undergo apoptosis.
Conclusion(s): Our study demonstrates the direct effects of drospirenone: decidualization of EuSC and reduced DNA synthesis of EcSC, but it does not cause EsSC apoptosis.

• 出版日期2015-7