Hesperetin Suppresses Inflammatory Responses in Lipopolysaccharide-Induced RAW 264.7 Cells via the Inhibition of NF-κB and Activation of Nrf2/HO-1 Pathways

作者：Ren, Hua; Hao, Jilong; Liu, Taotao; Zhang, Dongyan; Lv, Hongming; Song, E.^*; Zhu, Chao^*

来源：Inflammation, 2016, 39(3): 964-973.

DOI：10.1007/s10753-016-0311-9

• hesperetin
• inflammation
• NF-kappa B
• Nrf2
• HO-1

Hesperetin (Hesp), a common flavanone glycoside, was extracted from the fruit peel of Citrus aurantium L. (Rutaceae). Hesp has been shown to possess various biological properties, including antioxidant, neuroprotective, and anti-inflammatory properties. In this study, we investigated the protective effect of Hesp on inflammatory responses in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Our results indicated that Hesp treatment dramatically suppressed secretion of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-1 beta; reduced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) gene expression; inhibited NF-kappa B (p65) phosphorylation; and blocked I kappa B alpha phosphorylation and degradation. Further studies revealed Hesp markedly enhanced the heme oxygenase (HO)-1 and nuclear factor erythroid 2-related factor 2 (Nrf2) expression, which were involved with inducing Nrf2 nuclear translocation and decreasing Keap1 protein expression. Together, these results indicated that the anti-inflammatory effect of Hesp may be associated with NF-kappa B inhibition and Nrf2/HO-1 activation.

• 出版日期2016-6
• 单位吉林大学