An electrochemical sensing platform based on local repression of electrolyte diffusion for single-step, reagentless, sensitive detection of a sequence-specific DNA-binding protein

作者：Zhang, Yun^*; Liu, Fang; Nie, Jinfang; Jiang, Fuyang; Zhou, Caibin; Yang, Jiani; Fan, Jinlong; Li, Jianping

来源：Analyst, 2014, 139(9): 2193-2198.

DOI：10.1039/c4an00096j

• TRANSCRIPTION-FACTOR-BINDING
• LABEL-FREE DETECTION
• SMALL-MOLECULE
• BIOSENSORS
• THERAPY
• CANCER
• SENSOR

In this paper, we report for the first time an electrochemical biosensor for single-step, reagentless, and picomolar detection of a sequence-specific DNA-binding protein using a double-stranded, electrode-bound DNA probe terminally modified with a redox active label close to the electrode surface. This new methodology is based upon local repression of electrolyte diffusion associated with protein-DNA binding that leads to reduction of the electrochemical response of the label. In the proof-of-concept study, the resulting electrochemical biosensor was quantitatively sensitive to the concentrations of the TATA binding protein (TBP, a model analyte) ranging from 40 pM to 25.4 nM with an estimated detection limit of similar to 10.6 pM (similar to 80 to 400-fold improvement on the detection limit over previous electrochemical analytical systems).

• 出版日期2014
• 单位桂林理工大学