Decreased expression of long non-coding RNA WT1-AS promotes cell proliferation and invasion in gastric cancer

作者：Du, Tao; Zhang, Baogui; Zhang, Shun; Jiang, Xiaohua; Zheng, Ping; Li, Jianfang; Yan, Min; Zhu, Zhenggang; Liu, Bingya^*

来源：Biochimica et Biophysica Acta-Molecular Basis of Disease, 2016, 1862(1): 12-19.

DOI：10.1016/j.bbadis.2015.10.001

• WT1-AS
• Gastric cancer
• LncRNA
• Proliferation
• Progression

Tumor recurrence and metastasis remain the major obstacles for the successful treatment of patients diagnosed with gastric cancer. In recent years, long non-coding RNAs (lncRNAs) have been considered as key regulators of tumor behavior. In this study, we investigated the expression and biological role of a newly-identified cancer-related lncRNA, WT1-AS. We found that WT1-AS expression was significantly down-regulated in tumor tissues compared to matched adjacent non-tumor tissues. The WT1-AS expression level was also associated with tumor size and the clinicopathological stage. Cell proliferation, migration, and invasion were inhibited, and the proportion of G0/G1 cells increased when WT1-AS was ectopically-expressed in gastric cancer cells. Furthermore, ectopic expression of WT1-AS was demonstrated to inhibit tumor growth and metastasis in vivo. Finally, we found that WT1-AS overexpression could decrease ERK protein phosphorylation. Our study indicates that WT1-AS is significantly down-regulated in gastric cancers and may be correlated with tumor progression.

• 出版日期2016-1
• 单位上海交通大学; 同济大学; 济宁医学院; 上海市闵行区中心医院