alpha-Amylase and alpha-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grandis x E-urophylla Bark

作者：Jiang, Ping; Xiong, Jia; Wang, Fei; Grace, Mary H.; Lila, Mary Ann^*; Xu, Rui

来源：Journal of Chemistry, 2017, 2017: 8516964.

DOI：10.1155/2017/8516964

• PERFORMANCE LIQUID-CHROMATOGRAPHY
• ANTIOXIDANT ACTIVITY
• OUTER BARKS
• STEM BARK
• PHLOROTANNINS

This study evaluated the inhibitory effects of different extracts and fractions fromEucalyptus. grandis x urophylla bark (EB) against alpha-glucosidase and alpha-amylase enzyme activities. The ethyl acetate extract (EB-E) showed the highest activity among others. Seven fractions were derived from EB-E; among them EB-E-7 showed the highest significant inhibition of both enzymes, with IC50 of 1.40 +/- 0.18 and 1.72 +/- 0.12 mu g/mL, respectively. EB-E and its active fraction EB-E-7 showed highest contents of total phenolics: 178.79 +/- 4.68 and 920.4 +/- 5.46 mgGAE(a)g(-1), respectively. HPLC-MS analysis of EB-E-7 revealed the presence of ellagic acid, quercetin-glucuronide, quercetin-3- alpha-rhamnopyranoside, and ellagic acid rhamnoside as major compounds, together with smaller concentrations of myricetin-rhamnoside, isorhamnetin-hexoside, myricetin-3-alpha-arabinofuranoside, and isorhamnetin. Therefore, the phenolic compounds from Eucalyptus grandis x E. urograndis bark potently inhibited alpha-amylase and alpha-glucosidase activity, having potential in prevention of hyperglycemia.

• 出版日期2017
• 单位南京林业大学