A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells

作者：Huang, Chao; Zhu, Zhongkai; Cao, Xiyue; Chen, Xingfu; Fu, Yuping; Chen, Zhengli; Li, Lixia; Song, Xu; Jia, Renyong; Yin, Zhongqiong; Ye, Gang; Feng, Bin; Zou, Yuanfeng^*

来源：Molecules, 2017, 22(8): 1341.

DOI：10.3390/molecules22081341

• Sijunzi Decoction
• SJZD
• pectic polysaccharide
• oxidative stress
• antioxidant defense

Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysaccharides from SJZD are not well described. Here we obtained a purified polysaccharide (SJZDP-II-I) from SJZD using ion exchange chromatography and gel filtration. Structure analysis by FT-IR and NMR identified SJZDP-II-I as a typical pectic polysaccharide with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and II as side chains. In vitro studies indicated that SJZDP-II-I treatment could significantly enhance the total antioxidant capacity of SW480 cells, resulting from the promoted expressions of antioxidant enzymes and their master regulator PGC-1 alpha, which would be valuable for further research and applications.

• 出版日期2017-8
• 单位四川农业大学