Neuroglobin immunoreactivity in the human cochlea

作者：Vorasubin Nopawan; Hosokawa Seiji; Hosokawa Kumiko; Ishiyama Gail; Ishiyama Akira; Lopez Ivan A^*

来源：Brain Research, 2016, 1630: 56-63.

DOI：10.1016/j.brainres.2015.11.002

• Neuroglobin
• Human temporal bone
• Oxidative stress
• Inner ear
• Spiral ganglia neuron

Neuroglobin (Ngb) is an oxygen-binding protein with a demonstrated role in endogenous neuroprotective mechanisms. It has been shown to function as a scavenger for reactive oxidizing species thereby assisting in cellular defense against oxidative stress. In the present study, we characterized the presence of Ngb in the human cochlea. Immunohistochemical staining was performed on formalin fixed celloidin human cochlea sections obtained from human temporal bones, using affinity purified polyclonal antibodies against Ngb. Thirty-six temporal bones were analyzed, 15 with normal otologic histories and 21 diagnosed with different inner ear pathologies. Ngb immunoreactivity (Ngb-IR) was consistently expressed in the neurons of spiral ganglia (SG) and supporting cells of the organ of Corti. There was a significant decrease of Ngb-IR in SGNs from specimens with inner ear pathologies when compared to normal specimens. In contrast, Ngb-IR in the organ of Corti did not show significant changes between pathological and normal specimens. The differential pattern of Ngb expression in these cochlear structures suggests that Ngb may participate in defense mechanisms in inner ear pathologies where oxidative stress is involved.

• 出版日期2016-1-1