Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skin

作者：Feng, Weiwei; Zhao, Ting; Zhou, Ye; Li, Fang; Zou, Ye; Bai, Shiqi; Wang, Wei; Yang, Liuqing^*; Wu, Xiangyang

来源：Pharmacognosy Magazine, 2013, 9(36): 32-37.

DOI：10.4103/0973-1296.117859

• Extraction
• pepsin-soluble collagen
• response surface methodology
• sturgeon skin
• SDS-PAGE

Background: Sturgeon (Acipenser sturio Linnaeus) skin contains high amount of nutrients including unsaturated fatty acids and collagen. A pepsin-assisted extraction procedure was developed and optimized for the extraction of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skins. Objective: To determine the optimum conditions with the maximum yield of the pepsin-soluble collagen (PSC) extraction. Materials and Methods: The conditions of the extraction were optimized using response surface methodology. The Box-Behnken design was used to evaluate the effects of the three independent variables (extraction time, enzyme concentration, and solid-liquid ratio) on the PSC yield of the sturgeon skin. Results: The optimal conditions were: solid-liquid ratio of 1:11.88, enzyme concentration of 2.42%, and extraction time of 6.45 h. The maximum yield of 86.69% of PSC was obtained under the optimal conditions. This value was not significantly different from the predicted value (87.4%) of the RSM (P < 0.05). Conclusion: The results of this study indicated that the production of PSC from sturgeon skin is feasible and beneficial. The patterns of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns (SDS-PAGE) indicated that the sturgeon skin contains type I collagen, which is made of -chain and -chain. The infrared spectra of the collagens also indicated that pepsin hydrolysis does not affect the secondary structure of collagen, especially triple-helical structure.

• 出版日期2013-10
• 单位江苏大学