A strip-shield improves the efficiency of a solenoid coil in probes for high-field solid-state NMR of lossy biological samples

作者：Wu Chin H; Grant Christopher V; Cook Gabriel A; Park Sang Ho; Opella Stanley J^*

来源：Journal of Magnetic Resonance, 2009, 200(1): 74-80.

DOI：10.1016/j.jmr.2009.06.004

• Faraday shield
• Solenoid coil
• Double-resonance
• Membrane proteins

A strip-shield inserted between a high inductance double-tuned solenoid coil and the glass tube containing the sample improves the efficiency of probes used for high-field solid-state NMR experiments on lossy aqueous samples of proteins and other biopolymers. A strip-shield is a coil liner consisting of thin copper strips layered on a PTFE (polytetrafluoroethylene) insulator. With lossy samples, the shift in tuning frequency is smaller, the reduction in Q and RF-induced heating are all significantly reduced when the strip-shield is present. The performance of 800 MHz (1)H/(15)N and (1)H/(13)C double-resonance probes is demonstrated on aqueous samples of membrane proteins in phospholipid bilayers.

• 出版日期2009-9