MEIOB Targets Single-Strand DNA and Is Necessary for Meiotic Recombination

作者：Souquet Benoit^*; Abby Emilie; Herve Roxane; Finsterbusch Friederike; Tourpin Sophie; Le Bouffant Ronan; Duquenne Clotilde; Messiaen Sebastien; Martini Emmanuelle; Bernardino Sgherri Jacqueline; Toth Attila; Habert Rene; Livera Gabriel

来源：PLoS Genetics, 2013, 9(9): e1003784.

DOI：10.1371/journal.pgen.1003784

Meiotic recombination is a mandatory process for sexual reproduction. We identified a protein specifically implicated in meiotic homologous recombination that we named: meiosis specific with OB domain (MEIOB). This protein is conserved among metazoan species and contains single-strand DNA binding sites similar to those of RPA1. Our studies in vitro revealed that both recombinant and endogenous MEIOB can be retained on single-strand DNA. Those in vivo demonstrated the specific expression of Meiob in early meiotic germ cells and the co-localization of MEIOB protein with RPA on chromosome axes. MEIOB localization in Dmc1(-/-) spermatocytes indicated that it accumulates on resected DNA. Homologous Meiob deletion in mice caused infertility in both sexes, due to a meiotic arrest at a zygotene/pachytene-like stage. DNA double strand break repair and homologous chromosome synapsis were impaired in Meiob(-/-) meiocytes. Interestingly MEIOB appeared to be dispensable for the initial loading of recombinases but was required to maintain a proper number of RAD51 and DMC1 foci beyond the zygotene stage. In light of these findings, we propose that RPA and this new single-strand DNA binding protein MEIOB, are essential to ensure the proper stabilization of recombinases which is required for successful homology search and meiotic recombination.

• 出版日期2013-9
• 单位中国地震局