An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins

作者：Li Song; Koe Chwee Tat; Tay Su Ting; Tan Angie Lay Keng; Zhang Shenli; Zhang Yingjie; Tan Patrick; Sung Wing Kin; Wang Hongyan^*

来源：Nature Communications, 2017, 8(1): 122.

DOI：10.1038/s41467-017-00172-9

The switch between quiescence and proliferation is central for neurogenesis and its alteration is linked to neurodevelopmental disorders such as microcephaly. However, intrinsic mechanisms that reactivate Drosophila larval neural stem cells (NSCs) to exit from quiescence are not well established. Here we show that the spindle matrix complex containing Chromator (Chro) functions as a key intrinsic regulator of NSC reactivation downstream of extrinsic insulin/insulin-like growth factor signalling. Chro also prevents NSCs from ire-entering quiescence at later stages. NSC-specific in vivo profiling has dentified many downstream targets of Chro, including a temporal transcription factor Grainy head (Grh) and a neural stem cell quiescence-inducing factor Prospero (Pros). We show that spindle matrix proteins promote the expression of Grh and repress that of Pros in NSCs to govern their reactivation. Our data demonstrate that nuclear Chro critically regulates gene expression in NSCs at the transition from quiescence to proliferation.

• 出版日期2017-7-25