Molecular Basis of Bcl-X-L-p53 Interaction: Insights from Molecular Dynamics Simulations

作者：Bharatham Nagakumar^*; Chi Seung Wook; Yoon Ho Sup

来源：PLos One, 2011, 6(10): e26014.

DOI：10.1371/journal.pone.0026014

Bcl-X-L, an antiapoptotic Bcl-2 family protein, plays a central role in the regulation of the apoptotic pathway. Heterodimerization of the antiapoptotic Bcl-2 family proteins with the proapoptotic family members such as Bad, Bak, Bim and Bid is a crucial step in the apoptotic regulation. In addition to these conventional binding partners, recent evidences reveal that the Bcl-2 family proteins also interact with noncanonical binding partners such as p53. Our previous NMR studies showed that Bcl-X-L: BH3 peptide and Bcl-X-L: SN15 peptide (a peptide derived from residues S15-N29 of p53) complex structures share similar modes of bindings. To further elucidate the molecular basis of the interactions, here we have employed molecular dynamics simulations coupled with MM/PBSA approach. Bcl-XL and other Bcl-2 family proteins have 4 hydrophobic pockets (p1-p4), which are occupied by four systematically spaced hydrophobic residues (h1-h4) of the proapoptotic Bad and Bak BH3 peptides. We observed that three conserved hydrophobic residues (F19, W23 and L26) of p53 (SN15) peptide anchor into three hydrophobic pockets (p2-p4) of Bcl-X-L in a similar manner as BH3 peptide. Our results provide insights into the novel molecular recognition by Bcl-X-L with p53.

• 出版日期2011-10-19
• 单位南阳理工学院