A NOVEL GENE-PRODUCT ASSOCIATED WITH MU-CHAINS IN IMMATURE B-CELLS

作者：SHIRASAWA T; OHNISHI K; HAGIWARA S; SHIGEMOTO K; TAKEBE Y; RAJEWSKY K; TAKEMORI T

来源：The EMBO Journal, 1993, 12(5): 1827-1834.

• B-CELL MATURATION
• IMMUNOGLOBULIN SUPERGENE FAMILY
• MU-CHAIN COMPLEX
• MU-CHAIN EXPRESSION
• SURROGATE LIGHT CHAINS

A previously unreported B cell specific gene, which we have named 8HS-20, was isolated from the cDNA library of a pre-B cell clone by subtraction and differential hybridization. This gene is selectively expressed as a 0.75 kb transcript in pre-B and bone marrow-derived B cell lines; a transcript of the same size is also found in bone marrow and, albeit at low levels, in spleen. The deduced amino acid sequence of the 8HS-20 cDNA displayed homology to a B cell specific gene, VpreB-1, and to members of the immunoglobulin supergene family including Vlambda, Vchi, V(H), TCRValpha, Vbeta and CD8. Biochemical analysis using purified antiserum against 8HS-20 oligopeptides indicates that the gene encodes proteins with mol. wts of 13.5, 14, 15.5 and 16 kDa, which associate with mu chains in pre-B cell lines, and that these molecules are expressed concomitantly with VpreB-1 and lambda5 gene products in the same cell lines.

• 出版日期1993-5