Determinants of the Detection Limit and Specificity of Surface-Based Biosensors

作者：Esteban Fernandez de Avila Berta; Watkins Herschel M; Pingarron Jose M; Plaxco Kevin W; Palleschi Giuseppe; Ricci Francesco^*

来源：Analytical Chemistry, 2013, 85(14): 6593-6597.

DOI：10.1021/ac4012123

Here, we employ a model electrochemical DNA sensor to demonstrate that the detection limit and specificity of surface-based sensors often are not dependent on the true affinity of the probe for its target but are simply dependent on the effective probe concentration. Under these circumstances, the observed affinity (and thus the sensor%26apos;s detection limit and specificity) will depend on the density with which the probes are packed on the surface of the sensor, the surface area, and even the volume of sample employed.

• 出版日期2013-7-16