Apolipoprotein B100 analysis in microchip with electrochemical detection

作者：Liu, Cheng Cheng; Liu, Yun; Wang, Hui Xiang; Qi, Yan Bo; Yang, Peng Yuan; Liu, Bao Hong^*

来源：Chinese Chemical Letters, 2011, 22(11): 1343-1346.

DOI：10.1016/j.cclet.2011.05.028

• Microchip
• ApoB-100
• Immunoassay
• Electrochemical detection

Apolipoprotein B100 (apoB-100) is a major protein of the cholesterol-rich low-density lipoprotein (LDL) and reflects a better assessment of total atherogenic burden to the vascular system than LDL. In this work, a simple and sensitive method has been developed to determine picoliter apoB-100s using the PMMA microfluidic chip coupled with electrochemical detection system. This method performs very well with a detectable linear range of 1-800 pg/mL and a detection limit of 1 pg/mL. A real serum sample has further been detected by this microchip-based biosensor. The results show that this kind of method is practicable and has the potential application in clinical analysis and diagnosis.

• 出版日期2011-11
• 单位复旦大学