A novel system for cloning human TCRs Cutting short the way to TCR-based anticancer therapy

作者：Kobayashi Eiji; Kishi Hiroyuki^*; Muraguchi Atsushi

来源：OncoImmunology, 2014, 3(1): e27258.

DOI：10.4161/onci.27258

• gene therapy
• hTEC10
• single-cell RT-PCR
• T-cell receptor

T-cell receptor (TCR)-based gene immunotherapy has emerged as a promising approach for the treatment of multiple malignancies. We have recently reported an efficient system for the cloning and functional evaluation of TCR-coding cDNAs. This system, which we named hTEC10, allows for the determination of TCR antigen specificity in less than 10 days, and may therefore constitute a fast and powerful platform for the development of new TCR-based anticancer therapies.

• 出版日期2014-1