Alteration of Tumor Metabolism by CD4+T Cells Leads to TNF-alpha-Dependent Intensification of Oxidative Stress and Tumor Cell Death

作者：Habtetsion, Tsadik; Ding, Zhi-Chun; Pi, Wenhu; Li, Tao; Lu, Chunwan; Chen, Tingting; Xi, Caixia; Spartz, Helena; Liu, Kebin; Hao, Zhonglin; Mivechi, Nahid; Huo, Yuqing; Blazar, Bruce R.; Munn, David H.; Zhou, Gang^*

来源：Cell Metabolism, 2018, 28(2): 228-+.

DOI：10.1016/j.cmet.2018.05.012

• CD4(+) T-CELLS
• CHIMERIC ANTIGEN RECEPTOR
• NECROSIS FACTOR TNF
• ANTITUMOR FUNCTION
• CANCER-CELLS
• THERAPY
• ROS
• IMMUNOTHERAPY
• MICROENVIRONMENT
• CYCLOPHOSPHAMIDE

The inhibitory effects of cancer on T cell metabolism have been well established, but the metabolic impact of immunotherapy on tumor cells is poorly under-stood. Here, we developed a CD4+ T cell-based adoptive immunotherapy protocol that was curative for mice with implanted colorectal tumors. By conducting metabolic profiling on tumors, we show that adoptive immunotherapy profoundly altered tumor metabolism, resulting in glutathione depletion and accumulation of reactive oxygen species (ROS) in tumor cells. We further demonstrate that T cell-derived tumor necrosis factor alpha (TNF-alpha) can synergize with chemotherapy to intensify oxidative stress and tumor cell death in an NADPH (nicotinamide adenine dinucleotide phosphate hydrogen) oxidase-dependent manner. Reduction of oxidative stress, by preventing TNF-alpha-signaling in tumor cells or scavenging ROS, antagonized the therapeutic effects of adoptive immunotherapy. Conversely, provision of pro-oxidants after chemotherapy can partially recapitulate the antitumor effects of T cell transfer. These findings imply that reinforcing tumor oxidative stress represents an important mechanism underlying the efficacy of adoptive immunotherapy.

• 出版日期2018-8-7
• 单位宁夏医科大学