MTOC translocation modulates IS formation and controls sustained T cell signaling

作者：Martin Cofreces Noa B; Robles Valero Javier; Cabrero J Roman; Mittelbrunn Maria; Gordon Alonso Monica; Sung Ching Hwa; Alarcon Balbino; Vazquez Jesus; Sanchez Madrid Francisco^*

来源：The Journal of Cell Biology, 2008, 182(5): 951-962.

DOI：10.1083/jcb.200801014

T he translocation of the microtubule-organizing center (MTOC) toward the nascent immune synapse (IS) is an early step in lymphocyte activation initiated by T cell receptor (TCR) signaling. The molecular mechanisms that control the physical movement of the lymphocyte MTOC remain largely unknown. We have studied the role of the dynein-dynactin complex, a microtubule-based molecular motor, in the process of T cell activation during T cell antigen presenting cell cognate immune interactions. Impairment of dynein-dynactin complex activity, either by overexpressing the p50-dynamitin component of dynactin to disrupt the complex or by knocking down dynein heavy chain expression to prevent its formation, inhibited MTOC translocation after TCR antigen priming. This resulted in a strong reduction in the phosphorylation of molecules such as zeta chain associated protein kinase 70 (ZAP70), linker of activated T cells (LAT), and Vav1; prevented the supply of molecules to the IS from intracellular pools, resulting in a disorganized and dysfunctional IS architecture; and impaired interleukin-2 production. Together, these data reveal MTOC translocation as an important mechanism underlying IS formation and sustained T cell signaling.

• 出版日期2008-9-8