Comparison of 432 Pseudomonas strains through integration of genomic, functional, metabolic and expression data

作者：Koehorst Jasper J; van Dam Jesse C J; van Heck Ruben G A; Saccenti Edoardo; dos Santos Vitor A P Martins; Suarez Diez Maria^*; Schaap Peter J

来源：Scientific Reports, 2016, 6(1): 38699.

DOI：10.1038/srep38699

Pseudomonas is a highly versatile genus containing species that can be harmful to humans and plants while others are widely used for bioengineering and bioremediation. We analysed 432 sequenced Pseudomonas strains by integrating results from a large scale functional comparison using protein domains with data from six metabolic models, nearly a thousand transcriptome measurements and four large scale transposon mutagenesis experiments. Through heterogeneous data integration we linked gene essentiality, persistence and expression variability. The pan-genome of Pseudomonas is closed indicating a limited role of horizontal gene transfer in the evolutionary history of this genus. A large fraction of essential genes are highly persistent, still non essential genes represent a considerable fraction of the core-genome. Our results emphasize the power of integrating large scale comparative functional genomics with heterogeneous data for exploring bacterial diversity and versatility.

• 出版日期2016-12-6