Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs

作者：Xiaoyu, Pan; Desheng, Gong; Fei, Gao; Per Torp, Sangild

来源：Epigenomics, 2018, 10(4): 395-408.

DOI：10.2217/epi-2017-0122

<jats:p> Aim: To examine how enteral feeding affects the intestinal epigenome and gene expression just after preterm birth. Materials &amp; methods: Intestinal tissue from preterm pigs, modeling preterm infants, was collected at birth and 5 days after gradual introduction of infant formula or bovine colostrum. The intestinal tissue was analyzed by reduced representation bisulfite sequencing and real-time qPCR. Results: Relative to colostrum, formula increased bacterial epithelial adherence and lipopolysaccharide binding protein (LBP) expression, which was regulated by promoter methylation. Diet-dependent changes in DNA methylation and/or mRNA expression were related to innate immune response, hypoxia, angiogenesis and epithelial–mesenchymal transition pathways (e.g., TTC38, IL8, C3, HIF1A and VEGFR1). Conclusion: Epigenetic changes may mediate important effects of the first feeding on intestinal development in preterm neonates. </jats:p>

• 出版日期2018-4-1
• 单位中国农业科学院