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摘要
Background. Thirty-six extracts were obtained from nine plant raw materials and investigated for phenolic compound content, antiradical activity, and antimicrobial activity.
Objectives. The aim of this study was to investigate the antiradical activity of plant raw materials and extracts from these plants with a new method based oil using defined antiradical activity units and to study the antimicrobial activities of these extracts.
Material and Methods. Methanol water (1: 1) extract was obtained from the plant raw materials. Part of the extract was concentrated to dryness under reduced pressure (extract WA). Methanol was evaporated from the remaining extract and the precipitate separated (extract WD). An aqueous solution was extracted with ethyl acetate after precipitate separation. The ethyl acetate extract and aqueous solution remaining after ethyl acetate extraction was concentrated to dryness under reduced pressure to obtain extracts WB and WC, respectively. The dry extracts (WA, WB, WC, and WD) were investigated for antiradical activity (DPPH radical method) and for antimicrobial activity (disk-diffusion). A method of presenting antiradical activity in defined activity units is proposed. The amounts of phenolic compounds were measured with reagent containing phosphotungustic acid.
Results. The highest antiradical activity was measured for the extracts from oak bark, common bistort rhizome, and silverweed herb and the lowest for those from black cumin seed, ginseng root, and Siberian ginseng root. The strongest antibacterial activity (MIC) was observed for extracts from oak bark. The extract from black cumin seed also exhibited antifungal activity.
Conclusions. Raw materials such as oak bark, silverweed herb, and common bistort rhizome exhibited the strongest antiradical features. Among investigated raw materials only oak bark exhibited an interesting antimicrobial activity against bacterial as well as fungal strains. The use of antiradical activity units is convenient for estimating the yield of extraction of substances with antiradical activity (Adv Clin Exp Med 2008, 17, 3, 275-283).
- 出版日期2008-6
