NP/HILIC-ELSD Separation of Phospholipid Classes and Application to Preliminary Analysis of Plasma Low Density Lipoproteins

作者：Godoy Ramos Ricardo^*; Novoa Gundel Pedro; Jara Vasquez Pablo; Lamperti Fernandez Liliana; Gomez Gaete Carolina

来源：Journal of Liquid Chromatography & Related Technologies, 2015, 38(2): 243-250.

DOI：10.1080/10826076.2014.903849

• HILIC
• full factorial design
• lysophosphatidylcholine
• ELSD
• low density lipoproteins (LDL)
• normal phase
• phospholipid classes

A new method for the analysis of phospholipids (Lysophosphatidylethanolamine, Phosphatidylethanolamine, Phosphatidylcholine, Sphingomyelin, and Lysophosphatidylcholine) by NP/HILIC-ELSD is described using a silica column. Addition of formic acid and triethylamine to a mobile phase based on acetonitrile/methanol/water delivered isocratically promoted a good and rapid separation of 5 phospholipid classes (15min run). The use of an evaporative light scattering detector allowed the direct analysis of a mixture of phospholipids without complex sample pretreatment. Both power and linear regression showed satisfactory concordance with the relationship between response and concentration. The LOD and LOQ obtained were below 10ng and 30ng of injected mass for all cases, respectively. The method was validated with a standard mixture of phospholipids. This method has been applied to primarily characterize the phospholipid fraction of both native and oxidized low density lipoproteins.

• 出版日期2015-1-20