Antibody-free detection of infectious bacteria using quantum dots-based barcode assay

作者：Cihalova Kristyna; Hegerova Dagmar; Jimenez Ana Maria; Milosavljevic Vedran; Kudr Jiri; Skalickova Sylvie; Hynek David; Kopel Pavel; Vaculovicova Marketa; Adam Vojtech

来源：Journal of Pharmaceutical and Biomedical Analysis, 2017, 134: 325-332.

DOI：10.1016/j.jpba.2016.10.025

• Staphylococcus aureus
• Methicillin-resistant Staphylococcus aureus
• Klebsiella pneumoniae
• Magnetic particles
• Barcode
• Quantum dots

Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the most representative bacteria causing infectious diseases. Due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. Therefore, a sensitive determination of these pathogens is crucial for effective treatment.
The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from properties of magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detect as low concentrations of bacteria as 102 CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).

• 出版日期2017-2-5