Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

作者：Inacio Hanoch S M; Bomfim Maria Rosa Q; Franca Rafaela O; Farias Luiz M; Carvalho Maria Auxiliadora R; Serufo Jose Carlos; Santos Simone Goncalves^*

来源：Chemotherapy, 2014, 60(1): 54-62.

DOI：10.1159/000365726

• Pseudomonas aeruginosa
• Resistance markers
• Genetic diversity
• Bloodstream infections

Background: Pseudomonas aeruginosa commonly causes nosocomial bloodstream infections and the emergence of a variety of beta-lactarnases (BLs) is worrying. In 5 hospitals in Belo Horizonte, Brazil, the presence of phenotypes encoding BL genes was established and the genetic diversity of the P. aeruginosa strains recovered from bloodstream infections was analyzed. Materials and Methods: The isolates were investigated using a disk diffusion (DD) method and the Etest (R), for encoding nnetallo-beta-lactamases (MBLs), oxacillinases and cephalosporinases. Genes and genetic diversity were evaluated by random amplified polymorphic DNA (RAPD) genotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: Twelve strains (30%) were positive for MBLs by Etest and DD, 15 were cephalosporinase-positive and 87.5% were positive for bla(SPM-1) and bla(VIM-1). Twenty-three strains (57.5%) were grouped into profile A, 32.5% into profile B and 10% into profile C by RAPD genotyping. ERIC-PCR revealed a varying degree of similarity between strains, ranging from 45 to 100%. Conclusions:The results suggest distinct clonal populations in the 5 hospitals studied, indicating a potentially problematic epidemiological situation in Belo Horizonte, Brazil.

• 出版日期2014