Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1

作者：Fagundes Silva Joao Marcos; Al Rwahnih Maher; Blawid Rosana; Nagata Tatsuya; Martins Fajardo Thor Vinicius

来源：Virus Genes, 2017, 53(4): 667-671.

DOI：10.1007/s11262-017-1470-y

• High-throughput sequencing
• Virus discovery
• Luteoviridae
• Grapevine enamovirus-1
• GEV-1

In this study, we describe a novel putative Enamovirus member, Grapevine enamovirus-1 (GEV-1), discovered by high-throughput sequencing (HTS). A limited survey using HTS of 17 grapevines (Vitis spp.) from the south, southeast, and northeast regions of Brazil led to the detection of GEV-1 exclusively on southern plants, infecting four grapevine cultivars (Cabernet Sauvignon, Semillon, CG 90450, and Cabernet franc) with a remarkable identity of around 99% at the nucleotide level. This novel virus was only detected in multiple-virus infected plants exhibiting viral-like symptoms. GEV-1 was also detected on a cv. Malvasia Longa by RT-PCR. We performed graft-transmissibility assays on GEV-1. The organization, products, and cis-acting regulatory elements of GEV-1 genome are also discussed here. The near complete genome sequence of GEV-1 was obtained during the course of this study, lacking only part of the 3' untranslated terminal region. This is the first report of a virus in the family Luteoviridae infecting grapevines. Based on its genomic properties and phylogenetic analyses, GEV-1 should be classified as a new member of the genus Enamovirus.

• 出版日期2017-8