科研之友
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摘要
A method for estimating the positional displacement of protein bound gold nanoparticles is presented and used to estimate the rigidity of linkage of Au-144 nanoparticles bound to a tetrameric model protein. We observe a distribution of displacement values where most Au-144 clusters are immobilized to within 3 angstrom relative to the protein center of mass. The shape of the distribution suggests two physical processes of thermal motion and protein deformation. The application of this and similar rigid gold nanoparticle/protein conjugates in high resolution single particle electron cryo-microscopy is discussed.
- 出版日期2010-9-30
