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摘要
The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4\^7\_5\^9. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein(sorcin). Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.
- 出版日期2005
- 单位实验血液学国家重点实验室; 中国医学科学院血液学研究所; 中国科学院长春应用化学研究所; 吉林大学
