Mitochondrial reactive oxygen species generation triggers inflammatory response and tissue injury associated with hepatic ischemia-reperfusion: Therapeutic potential of mitochondrially targeted antioxidants

作者：Mukhopadhyay Partha; Horvath Bela; Zsengeller Zsuzsanna; Batkai Sandor; Cao Zongxian; Kechrid Malek; Holovac Eileen; Erdelyi Katalin; Tanchian Galin; Liaudet Lucas; Stillman Isaac E; Joseph Joy; Kalyanaraman Balaraman; Pacher Pal^*

来源：Free Radical Biology and Medicine, 2012, 53(5): 1123-1138.

DOI：10.1016/j.freeradbiomed.2012.05.036

• Ischemia-reperfusion
• Antioxidants
• Oxidative stress
• Reactive oxygen and nitrogen species
• Free radicals

Mitochondrial reactive oxygen species generation has been implicated in the pathophysiology of ischemia-reperfusion (I/R) injury; however, its exact role and its spatial-temporal relationship with inflammation are elusive. Herein we explore the spatial-temporal relationship of oxidative/nitrative stress and inflammatory response during the course of hepatic I/R and the possible therapeutic potential of mitochondrial-targeted antioxidants, using a mouse model of segmental hepatic ischemia-reperfusion injury. Hepatic I/R was characterized by early (at 2 h of reperfusion) mitochondrial injury, decreased complex I activity, increased oxidant generation in the liver or liver mitochondria, and profound hepatocellular injury/dysfunction with acute proinflammatory response (TNF-alpha, MIP-1 alpha/CCL3, MIP-2/CXCL2) without inflammatory cell infiltration, followed by marked neutrophil infiltration and a more pronounced secondary wave of oxidative/nitrative stress in the liver (starting from 6 h of reperfusion and peaking at 24 h). Mitochondrially targeted antioxidants, MitoQ or Mito-CP, dose-dependently attenuated I/R-induced liver dysfunction, the early and delayed oxidative and nitrative stress response (HNE/carbonyl adducts, malondialdehyde, 8-OHdG, and 3-nitrotyrosine formation), and mitochondrial and histopathological injury/dysfunction, as well as delayed inflammatory cell infiltration and cell death. Mitochondrially generated oxidants play a central role in triggering the deleterious cascade of events associated with hepatic I/R, which may be targeted by novel antioxidants for therapeutic advantage. Published by Elsevier Inc.

• 出版日期2012-9-1